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KMID : 1094719980030010024
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Biotechnology and Bioprocess Engineering
1998 Volume.3 No. 1 p.24 ~ p.31
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Separation and concentration of L-phenylalanine using a supported liquid membrane
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Choi Jeong-Woo
Cho Kyung-Sang
Youn In-Ju
Lee Won-Hong
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Abstract
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The separation and concentration of L-phenylalanine (L-Phe) using a supported liquid membrane (SLM) is investigated. A cation complex agent, di-2-ethylhexyl phosphoric acid (D2EHPA), is used as a carrier in the SLM with n-Heptane as a solvent. The reaction order and equilibrium constant in the formation reaction of L-Phe-carrier complex are obtained from the extraction experment. A mathematical model for a carrier mediated counter transport process is proposed to estimate the diffusion coefficient of L-Phe-carrier complex in the liquid membrane. Permeation experiments of L-Phe using a SLM are performed under various operating conditions and optimum conditions for the transport of L-Phe are obtained. Concentration of L-Phe in the strip phase against its concentration is observed. Transport rate of glucose through liquid membrane is less than that of L-Phe in the competitive transport of L-Phe and glucose. And the existence of glucose reduced the transport rate of L-Phe. The performance of separation with continuous strip phase is increased due to the dilution effect in the strip phase.
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KEYWORD
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supported liquid membrane, facilitated transport, L-Phe, glucose, D2EHPA
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